HPLC Separation of Amino Acids on Core-Shell Column in Reversed-Phase and Mixed-Mode. BLIS Separation of Amino Acids

Home HPLC Separation of Amino Acids on Core-Shell Column in Reversed-Phase and Mixed-Mode. BLIS Separation of Amino Acids

Get In Touch

Amino acids are very polar compounds which are used as a building blocks in pharmaceutical industry. They are widely used as supplements and food additives. Depending on the pH of the mobile phase they can exist in acidic, basic or zwitter-ionic form. At pH below 3, amino acids are basic in nature and have the highest hydrophobicity. Within pH of 3 to 7 amino acids are in zwitter-ionic form where they are the most hydrophilic. There is no mechanism of retention on reversed-phase column and amino acids are not retained. Bufferless ion-separation (BLIS) was introduced by SIELC as a way to retain and analyze amino acids in reversed-phase cation-exchange mode. This mode allows to analyze amino acids as zwitter-ions without any ions/buffers in the mobile phase. The method was adopted for analysis of amino acids on core-shell mixed-mode columns. A similar approach on a reversed-phase core-shell column results in distorted peaks and no separation or significant retention to achieve baseline separation.

Column Name : Coresep 100 Columns

Compound Name: Methionine, Phenylalanine, tyrosine

Get Your Quote or Call: 040-29881474

We focus on supporting laboratory workflows & optimizing lab-wide operations