HPLC Application Of SCX-HILIC Of Lung Surfactant Protein

If a protein isn’t normally found in an aqueous medium, such as a membrane protein, it may not be possible to do IEX at all without some organic solvent. The example below concerns Lung Surfactant Protein in an emulsion with 500 parts lipid. With 70% organic solvent in the mobile phase, hydrophilic interaction is superimposed on top of the electrostatic effects. Under these conditions, lipid is not retained and elutes in the void volume. The protein is then eluted with a salt gradient. A number of variants are evident, corresponding to truncation sequences with deletions from either terminus.

Column Name : PolyCAT A™ Columns

Compound Name : Lung Surfactant Protein